Genotypisierung (DNA)
The Genome Analysis Centre (GAC) of the Helmholtz-Zentrum Munich is run by the work group EpiBioGen (Supervisor: Dr. Thomas Illig) from the Institute of Epidemiology together with the Institute of Human Genetics at the Helmholtz-Zentrum Munich. A high-throughput genotyping platform has been established there, which makes up one of the genotyping centres of the National Genome Research Network (NFGN).
Genotyping is conducted via adequate and approved standard procedures (SOPs) and intern
quality controls. The genotyping centre uses a MALDI TOF MS system (matrix assisted
laser desorption / ionisation time of flight mass spectrometry system) by Sequenom
(Hamburg, San Diego). Genotyping is done in multiplex reactions and only small amounts
of genomic DNA are needed. Very large projects can be carried out in co-operation with
the Max-Plank Institute of Psychiatry (MPI, Munich-Schwabing) at an Illumina facility
(San Diego).
Also see: paper on genotyping in the list of
publications.
The categorisation of SNPs is accomplished by a fully automatic software program. The results of the genotyping are saved in a data bank and routinely checked for quality by means of negative controls, passive controls, duplicates, gender determination and verification of the Hardy-Weinberg equilibrium.
At the moment, a technical genotyping capacity of a total of 50.000 SNPs per day is available for KORA and other co-operative genotyping projects: in 2004 more than 5 million genotypes were transferred to different partners.
